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The invention relates to methods and devices enabling simultaneous detection of several biological threat agents, including viruses and bacteria. The device includes a plurality of chambers and conduits which can be manually operated to so that reagents and sample are passed through the device and nucleic acid hybridization membranes to permit detection by the naked eye. The device has minimal logistical requirements since it is self-contained and includes all the reagents required to process a sample suspected of containing a variety of biological threat agents, it does not require electrical or other external sources of energy, it is disposable, and it can operated by a soldier or responder without microbiological training or expertise.