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Processing microtitre plates for covalent immobilization chemistries

Patent image
NRL

Disclosed herein is a method of: treating an organic polymer with an electron beam-generated plasma; exposing the treated polymer to air or an oxygen- and hydrogen-containing gas, generating hydroxyl groups on the surface of the polymer; reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the surface than the chloro, fluoro, or alkoxy group; and covalently immobilizing a biomolecule to the functional or reactive group or a reaction product thereof.

Inventors: 
North, Stella H.; Lock, Evgeniya H.; Walton, Scott G.; Taitt, Chris Rowe
Patent Number: 
Technical domain: 
Chemical engineering
FIle Date: 
2010-11-17
Grant Date: 
2014-02-18
Grant time: 
1,189 days
Grant time percentile rank: 
21
Claim count percentile rank: 
5
Citations percentile rank: 
1
'Cited by' percentile rank: 
1
Assignee: 
US NAVY